描述
This reagent kit uses the Sequencing Based Typing method to type HLA genes, prepares amplification mixtures for HLA site specificity, and performs site-specific DNA amplification with Taq polymerase and sample genomic DNA. Enzymatic purification was performed on the amplified product to degrade residual primers and nucleotides, and then the purified PCR product was sequenced using site and exon specific sequencing primers. All products use BigDye fluorescent dye, which can provide users with efficient and accurate results and is recognized as the gold standard for HLA typing. The sequencing results were assisted by computer software to complete HLA allele typing.。
HLA-SBT High Resolution Typing Kits
The SBT HLA high-resolution sequencing kit from Beijing ROSE Genomics Corp . Ltd combined with a set of flexible additional 3SP primers, provides site specific amplification analysis capabilities to address current ambiguous results. Powerful allele recognition software makes genotyping easier and aims to provide reliable and consistent results.
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Product Introduction
HLA SBT Core Reagent Kit
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HLA SBT Experimental Process
Sequence specific sequencing primers
After the first sequencing and subsequent data analysis, ambiguous results may appear Once again, our company provides multiple 3S primers (sequence specific sequencing primers) specifically designed to address the most common ambiguous results.
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Easy to Set Up
★Unique primer design
★Ready to use PCR and sequencing mixes
★One thermocycler programme to amplify HLA-A,B,C,DR, DQ, and DP genes
★lf an ambiguity is present, the analysissoftware will suggest the appropriateSequence Specific Sequencing primer (3S-Primer)
Rapid
★Minimal hands-on-time to set upamplification and sequencing reactions
★High-speed purification of the PCR productachieves faster results
Accurate
★High resolution typing results of the HLA loci A,B,C,DR, DQ, and DP
★The ambiguities can be resolved with aflexible set of 3S-Primers
★Complete sequence of the clinicalrelevant exons is guaranteed in bothdirections
★lf required, sequencing primers foradditional exons can be provided
★Kits fulfill EFI requirements
HLA SBT Typing Kits Characteristics